Journal: International journal of molecular sciences

Article Title: Total Alkaloid Extract of Nelumbinis Plumula Promoted Sleep in PCPA-Induced Insomnia Rats by Affecting Neurotransmitters and Their Receptor Activities.

doi: 10.3390/ijms26083684

Figure Lengend Snippet: Figure 9. The effects of NP, NPA, and NPP extracts on GABRA1 and AChE proteins in hippocampus of PCPA-induced insomnia rats (n = 3) (magnification × 20, scale bar = 50 µm). (A) The effects in the CA1 area of hippocampus. (B) The effects in the CA3 area of hippocampus. (C) Quantification of the protein expression of GABRA1 in the CA1 area (x ± S) (n = 6). (D) Quantification of the protein expression of AChE in the CA1 area (x ± S) (n = 6). (E) Quantification of the protein expression of GABRA1 in the CA3 area (x ± S) (n = 6). (F) Quantification of the protein expression of AChE in the CA3 area (x ± S) (n = 6). Compared with the control group, #: p < 0.05; Compared with the model group, *: p < 0.05, **: p < 0.01, ***: p < 0.001.

Article Snippet: The GABRA1 (14471-1-AP) and DRD2 (55084- 1-AP) primary antibodies were purchased from Proteintech Group, Inc. (Wuhan, Hubei, China).

Techniques: Expressing, Control

Journal: CNS Neuroscience & Therapeutics

Article Title: Amphiregulin blockade decreases the levodopa‐induced dyskinesia in a 6‐hydroxydopamine Parkinson's disease mouse model

doi: 10.1111/cns.14229

Figure Lengend Snippet: AREG+ are highly expressed in dopamine 1 receptor (D1R) but not in dopamine 2 receptor (D2R). Using the double immunofluorescence to explore the co‐occurrence of AREG+/D1R+ or AREG+/D2R+, the findings indicate that AREG+ are markedly co‐expressed with D1R, but not D2R (Arrows in the upper figure). ( n = 3).

Article Snippet: For dual immunofluorescence, sections were incubated overnight at 4°C with either a mixture of dopamine 1 receptor (D1R) (cat # 17934‐1‐AP, RRID: AB 10598308, Proteintech) and AREG (cat # sc‐74501, RRID: AB 2227602, Santa Cruz) antibodies or dopamine 2 receptor (D2R) (cat # 55084‐1‐AP, RRID: AB 10859941, Proteintech) and AREG.

Techniques: Immunofluorescence

Journal: Acta neuropathologica communications

Article Title: Cholesterol-activated stress granules reduce the membrane localization of DRD2 and promote prolactinoma dopamine agonists resistance.

doi: 10.1186/s40478-025-01986-1

Figure Lengend Snippet: Fig. 4 Cholesterol promoted the formation of stress granules. (A) Plot of proteins identified via immunoprecipitation combined with mass spectrometry sequencing. (B-C) Functional annotation analysis of proteins with decreased or increased binding affinity to DRD2 after CHO/Mβ-CD treatment in MMQ cells. (D) Protein interaction network of proteins with increased binding affinity to DRD2 and the predominant ranks. (E) Correlation plot of G3BP1 and DRD2 expression in the pituitary gland according to the GTEx database. (F) Protein level of G3BP1 in whole-cell lysates (wcl) and P18000 pellets from MMQ cells. (G) Fluorescence staining of stress granules using TASG in MMQ cells. (H) Immunohistochemical staining of G3BP1 in tumor xenografts. (I) Western blot analysis of G3BP1 in tumor xenografts. (J) Immunohistochemical staining of G3BP1 in sensitive and resistant human prolactinomas. Scale bar: 5 μm

Article Snippet: After blocking with 5% skim milk mixed in TBST solution, the membranes were incubated with primary antibodies listed as follows, DRD2 (55084-1-AP, Proteintech, 1:1000), G3BP1 (66486-1-Ig, Proteintech, 1:1000), USP10 (19374-1-AP, Proteintech, 1:1000), Beta-Actin (81115-1-RR, Proteintech, 1:4000).

Techniques: Immunoprecipitation, Mass Spectrometry, Sequencing, Functional Assay, Binding Assay, Expressing, Fluorescence, Staining, Immunohistochemical staining, Western Blot

Journal: Acta neuropathologica communications

Article Title: Cholesterol-activated stress granules reduce the membrane localization of DRD2 and promote prolactinoma dopamine agonists resistance.

doi: 10.1186/s40478-025-01986-1

Figure Lengend Snippet: Fig. 5 Stress granules mediated the cholesterol-regulated reduction in DRD2 localization on the cell membrane. (A) USP10 can inhibit the assembly of stress granules, according to a previous study. (B) Overexpression of USP10 in MMQ cells and its effect on DRD2 protein levels. (C) Fluorescence staining of stress granules with TASG in USP10Mock and USP10OE MMQ cells. (D-E) Western blot analysis of the cell membrane proteins of USP10Mock and USP10OE MMQ cells. (F-G) Fluorescence staining of DRD2 in USP10Mock and USP10OE MMQ cells. (H-I) CCK-8 results of USP10Mock and USP10OE MMQ cells treated with CHO/Mβ-CD and cabergoline alone or in combination. (J) Fluorescence staining of stress granules with TASG in MMQ cells. (K) Western blot analysis of the levels of cell membrane proteins in MMQ cells. (L) CCK-8 results for MMQ-treated cells. *** P < 0.001, **** P < 0.0001. Scale bar: 5 μm

Article Snippet: After blocking with 5% skim milk mixed in TBST solution, the membranes were incubated with primary antibodies listed as follows, DRD2 (55084-1-AP, Proteintech, 1:1000), G3BP1 (66486-1-Ig, Proteintech, 1:1000), USP10 (19374-1-AP, Proteintech, 1:1000), Beta-Actin (81115-1-RR, Proteintech, 1:4000).

Techniques: Membrane, Over Expression, Fluorescence, Staining, Western Blot, CCK-8 Assay